Detection and Characterization of Bacterial Biofilms
Mon, Sep 16
2:00 PM — 3:15 PM
Steinman HallSteinman Hall 160 - Lecture Hall
Steinman Hall, 160 - Lecture Hall
The ChE Dept. would like to welcome Professor Edgar Goluch from Northeastern UniversityBacteria in nature typically exist as biofilms, where millions of individual cells, approximately 1 micrometer in diameter, are immobilized in a complex polymeric matrix that can be dozens of micrometers thick. Further, bacterial cells can undergo dramatic physical and biochemical changes, for example switching from a swimming to sessile state and encasing themselves in polysaccharides, in a matter of minutes. This wide range of size scales and rapid phenotypic changes require a unique set of approaches for quantitative analysis of bacterial species.
The Goluch Group investigates the chemical and physical micro-environments that bacteria respond to and create by developing and integrating nanoscale sensing elements inside microfabricated fluidic systems. This talk will describe the development of electrochemical sensors for bacterial analysis and real-time detection of infections. Of particular interest is Pseudomonas aeruginosa, a common, environmental, gram-negative bacteria that is one of the main causes of infection in ventilator-associated pneumonia, burn wound victims, and corneal inflammation among contact lens wearers. The use of surface plasmon resonance imaging (SPRi) as a new tool for studying biofilms will also be presented. SPRi provides high resolution imaging of surfaces up to 1 cm2 in seconds, allowing it to capture biofilm dynamics.
Professor Goluch received his B.S. in Chemical Engineering, M.S. in Mechanical Engineering, and Ph.D. in Bioengineering all from the University of Illinois at Urbana-Champaign. His graduate work involved development of Lab-on-a-Chip systems for clinical diagnostic applications. After that, he moved to the Kavli Institute of Nanoscience at the Delft University of Technology in the Netherlands as a National Science Foundation Postdoctoral Fellow. While there, he worked on single molecule detection techniques combining electrochemistry and nanofabrication. Professor Goluch is currently the DiPietro Assistant Professor in the Chemical Engineering Department at Northeastern University in Boston, MA. His current research interests are in the areas of biophysics and chemical sensors with a focus on bacterial biofilms.