Professor Anubhav Tripathi Seminar
Tue, Apr 09
2:00 PM — 3:15 PM
Steinman Hall160 Convent Avenue
Steinman Hall, 312
Professor Anubhav Tripathi SeminarTITLE: "On Developing Microfluidic Reactors for Diagnostic Applications "
ABSTRACT: We are developing several microfluidic routes to understand complex biomedical assays. Biomolecular transport, amplification reaction and sequence specific identification define some of the key aspects of these assays. We will give some examples to illustrate these aspects: We will focus on in vitro transcription reaction (IVT) of both a long and short strand of H5 influenza on both free and immobilized DNA templates to study the role of mass transfer and steric effects. We will show that transcription efficiency can be dramatically increased with optimal solution chemistry and steric effects due to surrounding immobilized molecules are only significant in certain specific conditions. We will present a microfluidic reactor for RNA amplification that uses mobilized components of binding reactions. This provides a basis for appropriate use of mass transfer and reaction arguments in successful application of amplification microreactors. We will also present the dispersion of molecules in a microchannel as they undergo a DNA amplification reaction that is driven by temporally changing temperatures. The results of this study enhance our understanding of transport in microscale systems in diverse areas relevant to micro-reactor design and DNA amplification.